Chromosomal organization is linked to the temporal and spatial control of gene expression in cellular development. Structuration of chromosomes is accomplished through a variety of intracellular protein-DNA interactions that are essential to the survival and functionality of the cell. The organization and positioning of the bacterial genome is controlled by a localization reaction called partition. Type I partition systems, the most widespread and the only ones present on chromosomes, centromere binding proteins, ParB, propagate along DNA from their nucleation at the centromere. In this work, we employ high-resolution ChIP-sequencing and epifluorescence microscopy to uncover the architecture of bacterial partition complexes.